Newsletter | June 23, 2021

06.23.21 -- Key Strategies For Intensifying Your Upstream Bioprocesses

 
Intensified And Improved Fed-Batch Production Process: High-Density N-1 Perfusion
 

Perfusion processes support high cell densities with retained exponential growth at high viability, which makes them interesting, not only for production processes, but also for seed train intensification. The method described here allows you to intensify your fed-batch process through reduced production duration without affecting the growth or titer profile.

A Model To Increase Yield In mAb Cell Culture Perfusion At Large Scale
 

As process intensification is adopted into large-scale manufacturing, the responsibility of scale-down models to accurately represent the expanded operating space quickly follows. Perfusion continues to be one such operating mode that lends itself to adding benefit at the manufacturing scale while adding complexity to the small-scale approach.

Introducing N-1 Perfusion To Intensify The Seed Train At A Higher Starting Density
 

N-1 perfusion refers to the intensification of the final seed train step by introducing perfusion. A perfusion process supplies the cell culture with a continuous flow of fresh media and removes spent media while retaining the cells in the bioreactor. A high-density N-1 seed culture can improve your fed-batch process by allowing you to seed the production (N) bioreactor at a higher starting density or to replace a large N-1 bioreactor.

Developing A Continuous Large-Scale Perfusion Cell Culture Process
 
Perfusion processes enable continuous operation over extended periods of time by constantly providing fresh nutrients for the cells and simultaneously removing spent media and waste products, as compared to batch and fed-batch processes. The key benefits of perfusion processes are compact process design, flexibility, increased productivity, increased yield, and consistent product quality.
A Simple, Three-Step Approach To Optimize The Fed-Batch Process For mAb Production
 
Developing and optimizing a new fed-batch process for mAb production does not have to be complicated. By learning some key things about your cell line from the start — the nutrients it needs over the culture time, impact of metabolites, and critical osmolality — you can quickly design a daily feeding regimen that is simple to perform at production scale.