Newsletter | June 9, 2021

06.09.21 -- Latest On Purifying Cryo-EM Samples And Infectious Virus Particles

 
Tips For Purifying Cryo-EM Samples
 

Cryogenic electron microscopy (cryo-EM) can help determine structures for challenging proteins, such as membrane proteins and larger complexes. The past years of technical improvements have made cryo-EM a very important and widely used technique within structural biology. This presentation provides tips on purifying your cryo-EM samples.

Purifying Infectious Virus Particles
 

Viruses are widely studied as pathogens and are extensively used as molecular tools and therapeutic agents. Making it easier to purify virus particles can help accelerate developments in virology. This application note demonstrates a chromatography method for purifying reovirus RNA virus particles using the ÄKTA™ start benchtop chromatography system, and HiTrap™ brand columns with Capto™ brand Core 700 resin.

Learn About Affinity Chromatography Using Tagged Protein Purification
 

Affinity tags can help you simplify your protein purification and protect your outcomes. Choosing the right tag is essential to getting the purity, solubility, and protein quality your application needs. Use this handbook to learn how to prepare a recombinant protein in the required quantity and quality.

Fundamentals Of Size Exclusion Chromatography
 

Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all chromatography techniques. SEC separates molecules by their differences in size as they pass through a resin packed in a column. You can use SEC for both preparative and analytical studies of your protein. But how do you know which parameters will give you the best resolution of your protein mixture?

Explore Protein Function Using Surface Plasmon Resonance (SPR)
 

Real-time monitoring of binding events using surface plasmon resonance (SPR) gives a deep understanding of the dynamics of molecular interactions. Learn how an academic research group used the Biacore™ X100 SPR system in their research to investigate protein-RNA, protein-protein interactions, and how researchers have used SPR to measure antibody specificity.