Boosting Cell Culture Yields With Intensified Fed Batch Processing

By Dongyoung Park, Minyong Eom, Hakyung Kim, and Jaewook Baek, Samsung Biologics

Intensified Fed-Batch (IFB) culture represents a significant advancement in biopharmaceutical manufacturing, addressing the limitations of traditional fed-batch (TFB) processes. By incorporating perfusion principles into the cell culture workflow, IFB enables higher cell densities, extended production phases, and improved product quality.

Key advantages of IFB culture include:

• Enhanced cell growth: The N-1 perfusion step provides a continuous supply of nutrients and removes inhibitory byproducts, fostering a healthy cell culture environment.
• Increased productivity: Higher cell densities and extended production phases lead to significantly higher product yields compared to TFB.
• Improved product quality: The controlled environment created by IFB culture contributes to consistent and high-quality product output.

Samsung Biologics' S-Tensify platform exemplifies the potential of IFB culture. By tailoring the process to specific cell lines and optimizing critical parameters, S-Tensify has achieved remarkable improvements in cell density, productivity, and product quality. This innovative approach has the potential to revolutionize biopharmaceutical manufacturing by enabling faster, more efficient, and cost-effective production of life-saving therapies.