Cytiva Cell datasheets catalogs app notes

  1. Recombinant Adeno-Associated Virus Type 5 Production Process 10/12/2022

    Learn about a scalable process for the production of rAAV using triple plasmid transfection of suspension cells in an animal-derived component-free medium.

  2. Apheresis Variability Control In Cell Therapy Manufacturing 7/25/2022

    Explore our best practices for apheresis collection and obtention of enriched product for cell therapies.

  3. Automating And Optimizing The Cell Harvesting Process 7/25/2022

    Discover how we automate the process of optimizing cell harvesting and further minimize the potential for cell loss during wash steps.

  4. Natural Killer Cells Manufacturing 7/22/2022

    Follow the feasibility of xeno‑free NK cell expansion, closure of key unit operations, and how future work could focus on fine‑tuning each step to generate a robust, scalable process.

  5. Optimizing Recovery On The Sepax™ Cell Processing System 7/21/2022

    Using a model T-cell line, we demonstrate the impact of varying rinse volume, intermediate volume, and manual extraction parameters on overall cell recovery with a final product dose volume of 20 mL.

  6. Genome Editing of Human Primary T Cells With Lipid Nanoparticles 4/18/2022

    We show CRISPR/Cas9-mediated knockouts of the T cell receptor and explore multi-step LNP engineering to produce TCRɑβ KO CAR T cells, a promising approach towards allogeneic CAR T cell therapy.

  7. Low-Volume Production For Screening High-Value Nanoparticle Materials 4/18/2022

    A platform that harnesses microfluidic mixing has been demonstrated as a simple, robust, and scalable production method for LNPs encapsulating various types of nucleic acids.

  8. Liquid Nitrogen-Free Cryopreservation For Sustainability 4/5/2021

    Discover the environmental implications of using liquid nitrogen for cell freezing, along with carbon emissions during the manufacture, transportation, and operation of LN2-free controlled-rate freezers.

  9. Upstream Platform For Continuous Lentiviral Vector Production 12/1/2020

    Here, we demonstrate a high-yield LV production process, using animal-derived component-free media and reagents, where multiple or continuous vector harvests could be obtained from a single batch.

  10. CAR-T Cell Lentiviral Transduction By Spinoculation 5/21/2020

    There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. Learn how to streamline this step while keeping flexibility during process development.